Raman micro-spectroscopy: theory and applications

16 Apr

Prof. Paola Sassi, Università degli Studi di Perugia

February 1-15, 2025

18 h, 3 CFU

The course aims to provide students with the tools necessary to use Raman spectroscopy in the microscopic characterization of different types of materials, from clays to cells. Starting from the description of the Resonant Raman and Raman effect (6 h), passing through the concepts that underlie microspectroscopy (2 h), the theoretical and experimental aspects of the most recent techniques of light scattering spectroscopy will be illustrated. In particular, the following techniques will be presented: SERS (Surface Enhanced Raman Scattering; 2 h); TERS (Tip Enhanced Raman scattering; 2 h); ROA (Raman Optical Activity; 2 h) and EDLS (Extended Depolarized Rayleigh Scattering; 2 h). A two-hour practice exercise will also give the students the possibility to analyze different samples with the micro-Raman instrumentation available in the “Molecular Spectroscopy” Lab of this Department.